论文部分内容阅读
人参为传统的名贵中药,其有效成分人参总甙。单体Rg1、Rb1对大脑皮质、脊背神经节(DRG)、交感节和脑干内神经细胞体外生长的影响已有报道。由于脊髓前角运动神经元的分离纯化较难实现,因此人参皂甙及其单体对它的作用目前并不清楚。本实验采用6.8％的甲泛影酰胺(Metrizamide)作梯度密度离心,从15d胚龄Wistar大鼠胚胎的脊髓内同时分离出胶角运动神经元
Ginseng is a traditional precious Chinese medicine, and its active ingredient is ginseng. The effects of monomers Rg1 and Rb1 on the in vitro growth of neurons in the cerebral cortex, dorsal ganglion (DRG), sympathetic joints, and brainstem have been reported. Because the separation and purification of spinal anterior horn motor neurons is difficult to achieve, the effects of ginsenosides and their monomers on it are not known. In this experiment, 6.8% of Metrizamide was used for gradient density centrifugation to simultaneously separate angulus motoneurons from the spinal cord of 15d embryonic Wistar rat embryos.