论文部分内容阅读
目的用不同种类、不同浓度的脂肪酸处理体外培养的3T3-L1成熟脂肪细胞,观察处理前后脂联素(ad-iponectin)及过氧化物酶体增殖物激活受体γ(PPARγ)基因mRNA的表达水平,及两者之间是否存在相互关系。方法运用实时荧光定量PCR方法检测体外培养并诱导分化成熟的3T3-L1脂肪细胞经一定浓度脂肪酸处理后,脂联素及PPARγ基因mRNA的表达水平。结果棕榈酸(PA)在低浓度(25μmol/L)时能明显上调脂联素及PPARγmRNA的表达,比对照组增加53%(P<0.05),其余浓度均呈表达下降;油酸(OA)和亚油酸(LA)则在浓度为25、50、100μmol/L时上调脂联素及PPARγmRNA的表达,在50μmol/L时上调作用最为明显,随着浓度的继续增加脂联素表达下降,呈剂量依赖关系,浓度越高,表达越低。结论油酸和亚油酸在一定浓度范围内上调3T3-L1脂肪细胞脂联素基因表达,棕榈酸则主要表现为抑制作用,但在很低浓度(25μmol/L)时也上调脂联素表达;脂联素基因表达与PPARγ基因表达相关。
Objective To observe the mRNA expression of adiponectin and peroxisome proliferator-activated receptor γ (PPARγ) mRNA in 3T3-L1 mature adipocytes cultured in vitro with different kinds and concentrations of fatty acids Level, and whether there is correlation between the two. Methods Real-time fluorescence quantitative PCR was used to detect mRNA expression of adiponectin and PPARγ in 3T3-L1 adipocytes cultured in vitro and induced by certain concentration of fatty acids. Results Palmitic acid (PA) significantly increased adiponectin and PPARγmRNA expression at a low concentration (25μmol / L), which was 53% more than that of the control group (P <0.05) And linoleic acid (LA) upregulated the expression of adiponectin and PPARγmRNA at concentrations of 25, 50 and 100μmol / L, upregulated at 50μmol / L. The expression of adiponectin decreased with the increase of concentration, In a dose-dependent manner, the higher the concentration, the lower the expression. CONCLUSION: Oleic acid and linoleic acid upregulate adiponectin gene expression in 3T3-L1 adipocytes at a certain concentration range. Palmitic acid mainly inhibits adiponectin expression, but it also up-regulates adiponectin expression at very low concentration (25μmol / L) ; Adiponectin gene expression and PPARγ gene expression.