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蘑菇母种的制作是其菌种生产过程中的第一阶段,也是最重要的阶段。母种的好坏直接影响到原种及栽培种的质量,也是影响栽培产量的主要因素之一。生产上用于蘑菇母种分离的方法主要有二种,即组织分离法和孢子分离法。组织分离法虽然操作简单,成功率高,但得到的母种菌丝生活力较差,而且经多代繁殖容易引起种性退化。孢子分离法能克服这两方面的不足,但存在一些技术上的难点。因担孢
The production of mushroom mother species is the first and the most important stage in the production of mushroom strains. The quality of the mother species directly affects the quality of the original species and the cultivated species, which is also one of the main factors that affect the yield of the cultivated species. There are two main methods for the separation of mushroom mother species in the production: tissue separation and spore separation. Though the method of tissue separation is simple and the success rate is high, the viability of the female parent mycelia is poor, and the degeneration of species is easy to occur after many generations of breeding. Spore separation can overcome these two shortcomings, but there are some technical difficulties. Due to Tamie