论文部分内容阅读
目的:观察通腑泄热法中药对兔肝部分切除术后肝功能的影响。方法:选用新西兰白兔25只，以随机数字表法随机分为3组，即中药组(TCM组，n n＝10)，对照组(Control组，n n＝10)，假手术组(Sham组，n n＝5)，制备兔肝部分切除模型20例和假手术模型(切开腹壁后关腹)5例，分别于术前、术后第1天、术后第3天、术后第5天，检测各组兔血清中过氧化氢酶(CAT)、丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、白蛋白(ALB)的含量水平。多组间样本比较采用单因素方差分析，组间两样本比较采用独立样本n t检验。n 结果:术前、术后第1天、术后第3天、术后第5天，中药组CAT的含量分别为(6.34±0.61)、(4.44±0.72)、(5.09±0.36)、(6.18±0.62) U/ml；对照组CAT的含量分别为(6.08±0.80)、(4.48±0.72)、(4.64±0.49)、(5.05±0.39) U/ml；假手术组CAT的含量分别为(6.34±0.64)、(6.31±0.52)、(6.33±0.42)、(6.36±0.31) U/ml。中药组ALT的含量分别为(25.67±2.97)、(38.65±3.14)、(33.30±2.33)、(26.95±1.74) U/L；对照组ALT的含量分别为(26.04±2.13)、(36.02±4.08)、(35.70±1.96)、(32.55±4.68) U/L；假手术组ALT的含量分别为(25.13±2.19)、(25.22±2.87)、(25.06±2.21)、(24.04±1.39) U/L。中药组AST的含量分别为(18.61±1.79)、(29.31±2.78)、(25.95±3.33)、(19.76±1.91) U/L；对照组AST的含量分别为(17.32±2.21)、(28.00±3.76)、(27.29±2.78)、(26.05±2.41) U/L；假手术组AST的含量分别为(18.27±2.15)、(18.92±2.41)、(18.87±1.89)、(18.54±2.28) U/L。中药组ALB的含量分别为(51.31±5.43)、(41.08±1.96)、(45.40±2.43)、(48.74±3.26) g/L；对照组ALB的含量分别为(52.14±4.36)、(42.99±4.16)、(45.33±3.71)、(43.74±2.95) g/L；假手术组ALB的含量分别为(50.05±4.23)、(51.18±4.11)、(51.10±2.93)、(51.50±3.34) g/L。CAT、ALT、AST、ALB的含量变化，术前各组间差异无统计学意义(CAT，n t＝0.815、-0.003、-0.633；ALT，n t＝-0.322、0.357、0.775；AST，n t＝1.443、0.325、-0.798；ALB，n t＝-0.377、0.451、0.881，n P值均>0.05)。术后第1天中药组和对照组ALT、AST水平明显升高，CAT、ALB水平明显下降，与假手术组比较，差异有统计学意义(ALT，n t＝8.017、5.257；AST，n t＝7.095、4.870；CAT，n t＝-5.119、-5.024；ALB，n t＝-6.589、-3.612，n P0.05)。术后第5天中药组和对照组ALT、AST都有所下降，CAT、ALB都有所升高，中药组变化更为显著，中药组和对照组间差异有统计学意义(ALT，n t＝-3.548；AST，n t＝-6.478；CAT，n t＝4.896；ALB，n t＝3.601，n P值均0.05). On the first day after operation, ALT and AST contents in TCM group and the control group were significantly increased, and CAT and ALB contents were significantly decreased. As compared with the sham operation group, the difference was statistically significant (ALT:n t=8.017, 5.257; AST: n t=7.095, 4.870; CAT: n t=-5.119, -5.024; ALB: n t=-6.589, -3.612, all n P0.05). On the 5th day after operation, ALT and AST contents decreased in the TCM group and the control group, and both CAT and ALB contents increased. The changes in the TCM group were more significant, and the difference between the TCM group and the control group was statistically significant (ALT:n t=-3.548; AST: n t=-6.478; CAT: n t=4.896; ALB: n t=3.601, all n P<0.05).n Conclusion:The Tongfu Xiere method can obviously reduce liver injury after partial hepatectomy in rabbits. It may increase the level of CAT and reduce the production of oxygen free radicals after partial hepatectomy, thereby protecting the liver function after partial hepatectomy.