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目的研究17-DMAG对缺氧模拟剂氯化钴(CoCl_2)诱导的人宫颈癌HeLa细胞的辐射敏感性影响。方法首先应用不同浓度的CoCl_2处理HeLa细胞24h,采用Western blot技术检测HIF-1α蛋白表达量,然后选取浓度为200μmol/L的CoGl_2对Hela细胞进行模拟缺氧处理24 h,应用不同浓度的17-DMAG预处理细胞16h后,接受2、4、6、8Gy不同剂量的射线照射,采用克隆形成实验观察细胞的存活率,应用Western blot技术检测HIF-1α蛋白表达。结果在CoCl_2浓度为200μmol/L时HIF-1a蛋白表达量最强;不同浓度17-DMAG处理组的HeLa细胞在不同照射剂量组间的存活率差异均有高度统计学论(均P<0.01);17-DMAG预处理缺氧HeLa细胞后,HIF-1a蛋白表达随药物浓度增加受到显著抑制。结论 17-DMAG预处理可增加缺氧细胞辐射敏感性,其机制可能是通过抑制HIF-1a蛋白表达而发挥作用。
Objective To investigate the effect of 17-DMAG on the radiosensitivity of human cervical carcinoma HeLa cells induced by cobalt chloride (CoCl2). Methods The HeLa cells were treated with different concentrations of CoCl 2 for 24 h. The expression of HIF-1α protein was detected by Western blotting. The Hela cells were treated with CoGl 2 at a concentration of 200 μmol / L for 24 h. Different concentrations of 17- After pretreated with DMAG for 16h, the cells were exposed to different doses of 2,4,6,8 Gy. The survival rate of cells was observed by clonogenic assay. The protein expression of HIF-1α was detected by Western blot. Results The expression of HIF-1a protein was the highest when the concentration of CoCl_2 was 200 μmol / L. The survival rates of HeLa cells treated with different concentrations of 17-DMAG were highly statistically significant (all P <0.01) After 17-DMAG preconditioning hypoxic HeLa cells, the expression of HIF-1a protein was significantly inhibited with the increase of drug concentration. Conclusion 17-DMAG preconditioning can increase the sensitivity of hypoxia cells to radiation. The mechanism may be through inhibiting the expression of HIF-1a protein.