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建立银翘散水煎液化学特征图谱并测定5种指标成分的含量,探索银翘散水煎液煎煮过程化学成分变化规律,为阐明银翘散“香气大出即取服”的科学内涵提供研究依据。制备不同煎煮时间银翘散水煎液,采用高效液相色谱法测定化学特征图谱及绿原酸、连翘苷、牛蒡苷、甘草苷、甘草酸含量,考察不同煎煮时间对银翘散水煎液化学成分的影响。结果显示,不同煎煮时间银翘散水煎液化学特征图谱相似度较大,表明所含化学成分组成类似;浸泡20 min后,银翘散5种指标成分溶出均达到2 h最大煎出量的39.7%;刚煮沸时,5种成分溶出均达到最大煎出量的69.5%;煮沸5 min时,香气大出,提示水煎液中挥发性成分浓度达到较大值,而5种成分溶出均达到最大煎出量的79.1%;煮沸10 min时,5种成分溶出均达到最大煎出量85.7%;煮沸15 min后,5种成分浓度变化均不明显(RSD<4.3%),且香气变淡,提示挥发性成分已大量蒸发散失。结果表明,煎煮时间对银翘散化学成分含量影响较大,煮沸约5 min时,香气大出,水煎液中挥发性和非挥发性成分浓度均较大,银翘散“香气大出即取服”传统煎煮方法具有一定的科学依据。
To establish the chemical characteristic map of Yinqiaosan decoction and to determine the content of five kinds of index components and to explore the chemical composition variation of decoction of Yinqiaosan decoction. To clarify the scientific connotation of Yinqiaosan Provide a research basis. Preparation of decocting time Yinqiaosan Decoction, the determination of chemical properties by high performance liquid chromatography and chlorogenic acid, forsythin, arctiin, glycyrrhizin, glycyrrhizic acid content, to explore different decoction of Yin Qiao San decoction Effect of liquid chemistry. The results showed that the similarity of the chemical characteristics of the decoction of Yinqiaosan at different decoctions was similar, indicating that the compositions of the chemical components contained were similar. After soaking for 20 min, the dissolution of five index components of Yinqian Powder reached the maximum decoction amount 39.7%. When boiling, all the five components reached 69.5% of the maximum amount, and when boiled for 5 minutes, the aroma was higher, indicating that the concentration of volatile components in the decoction reached a larger value. However, Reached 79.1% of the maximum amount of fried; boiling 10 min, the maximum amount of five components were eluted 85.7%; after 15 min boiling, the concentration of the five components were not significantly changed (RSD <4.3%), and the aroma change Light, suggesting that a large number of volatile components have evaporated. The results showed that the decocting time had a significant effect on the chemical composition of Yinqiao powder. When the boiling time was about 5 min, the aroma was large and the concentration of volatile and non-volatile components in the decoction was large. Out of that service. "Traditional decoction method has a certain scientific basis.