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Objective:To investigate the effects of 50% ethyl alcohol(EtOH)extracts from Danzhi Xiaoyao Pill(丹栀逍遥丸,DXP)on the proliferation of MCF-7 human breast cancer cells and potential mechanisms.Methods:ATP-Lite assay was performed to test the proliferation of the MCF-7 breast cancer cell line;and antioxidant activity was measured by the oxygen radical absorbance capacity (ORAC).The effects of DXP on nitric oxide(NO)production were tested by lipopolysaccharide(LPS)- stimulated RAW 264.7 murine macrophages using the Griess reaction.Results:The 50% EtOH DXP extracts displayed a cytotoxic response on MCF-7 cells at 0.10,0.25 and 0.50 mg/mL dose- dependently with the proliferation inhibited by more than 85%.The ORAC value of the DXP was 820μmoL Trolox equivalent/g,about 40% of the vitamin C value.DXP extracts had significant inhibitory effect on NO production at the concentration from 0.0625 mg/mL to 0.5 mg/mL(P<0.05,P<0.01).Conclusion:The extracts of DXP could significantly inhibit the proliferation of MCF-7 cells,with the effect possibly related to its antioxidant activity and the inhibition of NO production.
Objective: To investigate the effects of 50% ethyl alcohol (EtOH) extracts from Danzhi Xiaoyao Pill on the proliferation of MCF-7 human breast cancer cells and potential mechanisms. Methods: ATP-Lite assay was performed to test the proliferation of the MCF-7 breast cancer cell line; and antioxidant activity was measured by the oxygen radical absorbance capacity (ORAC). The effects of DXP on nitric oxide (NO) production were tested by lipopolysaccharide (LPS) 264.7 murine macrophages using the Griess reaction. Results: The 50% EtOH DXP extracts displayed cytotoxic response on MCF-7 cells at 0.10, 0.25 and 0.50 mg / mL dose- dependently with the proliferation inhibited by more than 85%. ORAC value of the DXP was 820 μmol L Trolox equivalent / g, about 40% of the vitamin C value. DXP extracts had a significant inhibitory effect on NO production at the concentration from 0.0625 mg / mL to 0.5 mg / mL (P <0.05, P <0.01) .Conlusion: The extracts of DXP could significantly inhibited th e proliferation of MCF-7 cells, with the effect possibly related to its antioxidant activity and the inhibition of NO production.