论文部分内容阅读
目的 探讨高强度聚焦超声 (highintensityfocusedultrasound ,HIFU)对恶性肿瘤局部组织中免疫状态的影响。方法 利用Wistar大鼠 ,采用癌组织块包埋法建立大鼠W2 5 6肝癌模型 (4 0只 ) ;同时 ,以相同程序建立正常肝对照组 (C组 ,大鼠 2 0只 )。 10d后 ,应用HIFU(10MHz,15 2 8 7W/cm2 ,2 0s× 2次 )对治疗组 (A组 ) 2 0只大鼠的癌灶进行照射 ;以同样方式照射C组大鼠相同部位的正常肝组织 ;荷癌肝对照组 (B组 )则按同样程序对 2 0只大鼠的癌灶进行假照处理。 2周后活杀动物取材。进行组织学观察 ,并用免疫组织化学方法对照射区周围局部组织中的T淋巴细胞亚群 (T lympho cytesubsets ,T LS)进行原位半定量观察。结果 光学显微镜下见靶区组织凝固坏死 ,无癌细胞或肝细胞残存。A组局部组织中CD4 +和CD8+细胞数量 (16 1± 6 3 ,12 0± 5 2 )个 ,较B组 (5 8± 3 0 ,8 3±4 7)个、C组 (1 7± 1 1,1 2± 0 6 )个显著增加 (P <0 0 0 1,P <0 0 5 ;P <0 0 0 1,P <0 0 0 1)。其中尤以CD4 +细胞增加明显 ,CD4 +/CD8+细胞比值大于 1;B组的CD4 +和CD8+细胞数量 (5 8± 3 0 ,8 3± 4 7)个亦明显高于C组的 (1 7± 1 1,1 2± 0 6 )个 (P <0 0 0 1,P <0 0 0 1)。但CD4 +/CD8+细胞比值 (0 7±0 3)小于 1;C?
Objective To investigate the effect of high intensity focused ultrasound (HIFU) on the immune status in local tissues of malignant tumors. Methods Wistar rats were used to establish a rat model of W256 liver cancer (40). Meanwhile, a normal liver control group (Group C, 20 rats) was established by the same procedure. After 10 days, the foci of 20 rats in the treatment group (group A) were irradiated with HIFU (10 MHz, 1528 7 W/cm2, 20 seconds × 2 times); the same part of the rats in group C was irradiated in the same manner. Normal liver tissue; cancer-bearing liver control group (B group) were treated according to the same procedure on the foci of 20 rats. After 2 weeks, animals were killed and killed. Histological observation was performed and immunohistochemical methods were used to observe the T lymphocyte subsets (T LS) in the local tissue surrounding the irradiated area in situ. Results Under the light microscope, tissue coagulation and necrosis was seen in the target area, and no cancer cells or hepatocytes remained. The number of CD4 + and CD8 + cells in the local tissue of group A (16 1 ± 6 3, 12 0 ± 5 2) was higher than that in group B (5 8 ± 3 0, 8 3 ± 4 7), and in group C (1 7 ± There was a significant increase in 1, 1, 2 ± 0 6 (P <0 0 0 1, P <0 0 5; P <0 0 0 1, P <0 0 0 1). Among them, the number of CD4 + cells increased significantly, and the ratio of CD4 +/CD8 + cells was greater than 1; the number of CD4 + and CD8 + cells in group B (5 8 ± 3 0, 8 3 ± 47) was also significantly higher than that of group C (1 7 ± 1 1, 1 2 ± 0 6) (P <0 0 0 1, P <0 0 0 1). However, the ratio of CD4 +/CD8 + cells (0 7 ± 0 3) is less than 1; C?