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已证实组织或细胞经过培养后抗原性减低，将培养组织或细胞移植到同种或异种受体均能成活。本研究从细胞表面糖链入手，通过测定ＢＡＬＢ／Ｃ小鼠垂体细胞培养前后末端糖唾液酸含量，以及培养前后垂体细胞的免疫原性改变，观察细胞表面唾液酸含量对其免疫原性的影响。结果发现培养后的垂体细胞唾液酸含量显著高于未培养细胞；细胞唾液酸含量增高后，其刺激异基因大鼠脾细胞增殖的能力降低。用唾液酸酶去除培养细胞表面部分唾液酸后，可大大恢复其刺激异基因脾细胞增殖的能力。提示培养细胞唾液酸表达增加是导致其免疫原性降低的重要分子学基础
It has been confirmed that the antigenicity of the tissue or the cell after the culture is reduced, and the cultured tissue or the cell can be transplanted to the same or the different type of the receptor. This study started from the cell surface sugar chain, by measuring the content of sialic acid before and after the culture of pituitary cells in BALB / C mice and the immunogenicity of pituitary cells before and after culture, the effect of sialic acid on the immunogenicity was observed . The results showed that the content of sialic acid in cultured pituitary cells was significantly higher than that in uncultured cells. The increased ability of sialic acid to stimulate proliferation of allogeneic rat splenocytes was decreased. After sialic acid is removed from the surface of cultured cells by sialidase, its ability to stimulate proliferation of allogenic splenocytes can be greatly restored. Suggesting that increased sialic acid expression in cultured cells is an important molecular basis for the reduction of immunogenicity