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探讨剔毒护肝方介导UEPG_2细胞凋亡的可能机制,方法:培养24h的HEPG_2细胞,分别给予剔毒护肝方(12.5mg/dl)及阿霉素(10μmol/ml)作用8d,同时设空白对照组,细胞凋亡采用流式细胞仪测定,Bcl-2、Bax、Fas采用S-P免疫组化法检测。结果:剔毒护肝方、阿霉素及空白对照组的凋亡率分别为24.43％、10.58％、2.05％。剔毒护肝方组细胞Bcl-2、Bax、Fas的表达量分别为0.118±0.015、0.152±0.028、0.121±0.018,与空白对照组及阿霉素组相比有显著性差异(P<0.01)。结论:剔毒护肝方有明显介导HEPG_2细胞凋亡的作用,并可能通过介导凋亡调控基因Pas、Pax的高表达及下调Bcl-2基因的水平来介导凋亡。
To explore the possible mechanism of Jiedu Hugan Recipe on apoptosis of UEPG-2 cells. Methods: HEPG-2 cells were cultured for 24 hours and given respectively Tiaodu Hugan Fang (12.5mg/dl) and Adriamycin (10μmol/ml) for 8 days. In the blank control group, apoptosis was measured by flow cytometry, and Bcl-2, Bax, and Fas were detected by SP immunohistochemistry. RESULTS: The apoptotic rates of Tiaodu Hugan Decoction, Adriamycin, and blank control group were 24.43%, 10.58%, and 2.05%, respectively. The expression levels of Bcl-2, Bax, and Fas in the Tiaodu Hugan Recipe group were 0.118±0.015, 0.152±0.028, and 0.121±0.018, respectively, which were significantly different from those in the blank control group and the doxorubicin group (P<0.01). ). Conclusion: Tiaodu Hugan Decoction can obviously mediate the apoptosis of HEPG-2 cells, and may mediate apoptosis through mediating the high expression of Pas, Pax and down-regulating the level of Bcl-2 gene.