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胶胞炭疽菌是引起红掌炭疽病的病原菌。根据GenBank中炭疽属不同种的ITS序列差异,设计了胶胞炭疽菌的特异性引物E1/E2,由此建立的PCR检测体系可以从38个胶胞炭疽菌菌株中扩增得到329 bp的特异性条带,而扩增其它近似或相关菌株时没有相应的特异性条带。该检测体系对胶胞炭疽菌基因组DNA的扩增灵敏度达到10 pg。将引物E1/E2与ITS区通用引物进行套式PCR扩增后,检测灵敏度至少提高10 000倍。当土中胶胞炭疽菌分生孢子达到200个/g土时可检测出。进一步利用此检测体系对携带病原菌的灌溉水、发病组织进行检测,均能快速稳定地检测出病原菌。
Colletocystis anthracnose is the pathogen causing anthracnose. According to the difference of ITS sequences of different anthrax species in GenBank, the specific primer E1 / E2 of Colletotrichum gloeosporioides was designed and the PCR detection system was established to amplify 329 bp from 38 strains of Colletotrichum gloeosporioides However, there is no corresponding specific band when amplifying other similar or related strains. The detection system of Colbottum anthrax genomic DNA amplification sensitivity of 10 pg. After the primers E1 / E2 and ITS primers were used for nested PCR amplification, the detection sensitivity increased by at least 10,000 times. When the soil cell colony anthrax conidia reached 200 / g soil can be detected. Further use of this detection system to carry pathogenic bacteria in irrigation water, the detection of pathogens, can quickly and stably detect pathogens.