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目的:通过分选人宫颈癌细胞中的侧群细胞验证其肿瘤干细胞特性。方法:取16例行宫颈鳞癌切除的手术标本进行宫颈癌细胞原代培养,流式细胞仪分选侧群细胞(Side Population,SP),对比SP细胞和非SP细胞(Non Side Population,Non-SP)的体外自我更新能力和肿瘤形成能力。结果:在原代培养的16株宫颈癌细胞中均分选出SP细胞,比例约占(1.67±0.94)%。SP细胞的克隆形成能力显著高于NSP细胞,SP细胞在裸鼠肿瘤形成能力显著高于NSP细胞。结论:人宫颈癌侧群细胞具有肿瘤干细胞特性,为宫颈癌干细胞的研究提供了简单、有效的分离方法。
Objective: To verify the characteristics of tumor stem cells by sorting side population cells in human cervical cancer cells. Methods: Sixteen patients with cervical squamous cell carcinoma were selected for primary culture of cervical cancer cells. Flow cytometry was used to sort side population (SP) cells. Non-SP cells were compared with non-SP cells -SP) in vitro self-renewal ability and tumor formation ability. Results: The SP cells were sorted out in the 16 primary cultured cervical cancer cells, accounting for about 1.67 ± 0.94%. The clonogenic capacity of SP cells was significantly higher than that of NSP cells. The ability of SP cells to form tumors in nude mice was significantly higher than that of NSP cells. CONCLUSION: Human cervical cancer colony cells have tumor stem cell characteristics and provide a simple and effective method for the isolation of cervical cancer stem cells.