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目的采用生物信息学技术预测人类微小RNA-409-3p(hsa-miR-409-3p)靶基因,并分析其可能参与的生物学过程及信号通路。方法应用miRbase数据库和UCSC Genome Browser分析工具获取hsa-miR-409-3p的染色体定位、碱基序列和物种保守性等基本信息,利用miRanda、miRDB、Target Scan和miRwalk进行靶基因预测,采用miRwalk网站对靶基因取交集,合并有文献支持和经实验证实的靶基因作为基因集,对基因集进行功能富集分析(GO分析)和信号通路分析。结果hsa-miR-409-3p序列在各物种间高度保守。对miRanda、miRDB、Target Scan和miRwalk预测的靶基因进行交集后共得到273个靶基因。miRwalk中检索到经验证的靶基因有133个。合并后进行GO分析,结果显示靶基因富集于细胞粘附、细胞基质粘附、生长调节、调节干细胞群维持、学习或记忆、突触传递的负调节等(P<0.05)。信号通路分析结果显示靶基因富集于PI3K-Akt信号通路、Hippo信号通路、AMPK信号通路、神经营养蛋白信号通路、B细胞受体信号通路、安非他明成瘾等(P<0.05)。结论 hsa-miR-409-3p参与神经系统功能及其调节的生物学过程,与神经系统疾病的发生密切相关。
Objective To predict the target gene of human microRNA-409-3p (hsa-miR-409-3p) using bioinformatics technology and analyze its possible biological processes and signal pathways. Methods The basic information of hsa-miR-409-3p, such as the chromosome location, nucleotide sequence and species conservation, was obtained using the miRbase database and UCSC Genome Browser analysis tools. The target genes were predicted by using miRanda, miRDB, Target Scan and miRwalk. The target genes were taken as a set, combined with literature support and experimentally confirmed target genes as a gene set, the gene set was subjected to functional enrichment analysis (GO analysis) and signal pathway analysis. Results The hsa-miR-409-3p sequence was highly conserved among all species. A total of 273 target genes were obtained after crossing the predicted target genes of miRanda, miRDB, Target Scan and miRwalk. There were 133 validated target genes retrieved in miRwalk. After the merger, GO analysis showed that the target genes were enriched in cell adhesion, cell matrix adhesion, growth regulation, regulation of stem cell population maintenance, learning or memory, and negative regulation of synaptic transmission (P <0.05). The signal pathway analysis showed that the target genes were enriched in PI3K-Akt signaling pathway, Hippo signaling pathway, AMPK signaling pathway, neurotrophin signaling pathway, B cell receptor signaling pathway, and amphetamine addiction (P <0.05). Conclusions hsa-miR-409-3p is involved in the biological process of nervous system function and regulation, which is closely related to the occurrence of nervous system diseases.