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目的：研究肾癌来源的肿瘤浸润淋巴细胞体外增殖能力与转化生长因子表达之间的关系。方法：自13例肾癌组织标本中分离新鲜的肿瘤浸润淋巴细胞，在含不同浓度的重组人白细胞介素-2的完全培养液中培养3周，了解其体外扩增能力。同时对肾癌组织标本作石蜡切片后，应用原位杂交技术检测肾癌组织中转化生长因子-β1mRNA的表达，对部分液氮冻存的肾癌组织，抽提其RNA，利用Slot印迹技术检测TGF－β1mRNA的表达。结果：在rhuIL－2浓度为100IU／ml和1000IU／ml的培养液中培养3周，TIL的扩增值数分别为x=112±117（范围：12～409）和x＝694±524（范围：59～1748）。其中TGF－β1mNA表达组，TIL的扩增值数分别为x＝30±11（范围：12～49）和x=221±128（范围：59～350），而TGF－β1mRNA不表达组，TIL的扩增值数分别为x＝183±121（范围：37～409）和x＝913±524（范围：91～1748）。结论：TIL随着培养液中IL－2的浓度增加，其扩增能力增强。TIL的扩增能力与肿瘤组织中TGF-β1mRNA的关系呈反向关系。
OBJECTIVE: To study the relationship between the proliferation of tumor-infiltrating lymphocytes derived from renal carcinoma and the expression of transforming growth factor in vitro. Methods: Fresh tumor infiltrating lymphocytes were isolated from 13 cases of renal cell carcinoma and cultured in complete medium containing different concentrations of recombinant human interleukin-2 for 3 weeks to understand their in vitro expansion ability. At the same time, the specimens of renal cell carcinoma were paraffin-embedded and the expression of transforming growth factor-β1 mRNA in renal cell carcinoma was detected by in situ hybridization. RNA was extracted from some of the renal carcinoma tissues frozen in liquid nitrogen and detected by Slot blotting TGF-β1 mRNA expression. Results: The number of expansion of TIL was x = 112 ± 117 (range: 12 to 409) and x = 694 ± 524 (3 to 6) in culture medium with rhuIL-2 concentrations of 100 IU / ml and 1000 IU / Range: 59 ~ 1748). The expression of TIL in TGF-β1mNA group and the TIL group were x = 30 ± 11 (range: 12-49) and x = 221 ± 128 (range: 59-350) The amplitudes were x = 183 ± 121 (range: 37-409) and x = 913 ± 524 (range: 91-1748), respectively. Conclusion: With the increase of IL-2 concentration in TIL, the TILs of the TILs are enhanced. The relationship between the ability of TIL to amplify and TGF-β1 mRNA in tumor tissue is inversely related.