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为了探索人乳头状瘤病毒(HPV)与食管癌发生的关系,对食管鳞癌、癌旁鳞状上皮及乳头状瘤进行组织病理学研究,并用生物素标记的HPV_(16)DNA探针进行原位杂交。结果:癌旁鳞状上皮有凹空细胞等HPV感染的组织形态学改变者为65.2%(15/23),HPV_(160)DNA检出率为56.5%(13/23);食管鳞癌有凹空样癌细胞者为27.8%(10/36),HPV_(16)DNA检出率为50.0%(18/36);6例乳头状瘤均检出HPV_(16)DNA(100.0%)。不同分化程度的食管鳞癌(Ⅰ、Ⅱ、Ⅲ级)HPV_(16)DNA检出率分别为100.0%(6/6),60.0%(9/15)和20.0%(3/15)。研究结果提示,HPV感染与食管鳞癌发生有关,并与其分化和某些组织学特征有关。
To investigate the relationship between human papillomavirus (HPV) and esophageal carcinogenesis, histopathological studies of esophageal squamous cell carcinoma, adjacent squamous cell carcinoma, and papilloma were performed and biotin-labeled HPV 16 DNA probes were used. In situ hybridization. RESULTS: The morphological changes of HPV-infected squamous epithelial cells were 65.2% (15/23). The detection rate of HPV_(160) DNA was 56.5% (13/23); esophageal squamous cell carcinoma had The rate of HPV_(16) DNA detection was found to be 27.0% (10/36) in the tumors with a hollow space-like appearance of 50.0% (18/36); HPV_(16) DNA was detected in 6 papillomas (100.0%). The detection rates of HPV 16 DNA in esophageal squamous cell carcinomas (I, II, III) with different degrees of differentiation were 100.0% (6/6), 60.0% (9/15), and 20.0% (3/15), respectively. The results suggest that HPV infection is associated with the occurrence of esophageal squamous cell carcinoma and is associated with its differentiation and certain histological features.