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用硫氰酸钾盐析法二次纯化人脐血干扰索,获得部分纯化干扰素(P-IFN)。抗病毒活性比粗制干扰素提高50倍左右,比活性为10~5单位毫克蛋白~(-1)。回收率约为37％,在纯化过程中损失较多。为了减少损失,除在操作过程中注意保冷外,并采取勤换透析液、缩短透析时间,用纤维素微孔滤膜代替蔡氏滤器,作了一些小的改进。经上进方法处理的P-IFN,效价无明显影响。用低浓度(1～3×10~4单位毫升~(-1))的P-IFN,局部注射球结膜下或浅眶内,治疗疱疹性角膜炎28例,治愈好转率为92.9％,尤以上皮型疗效最佳,治愈率为100％。此种方法具有疗效高、反应小、干扰素用量少等优点,有利于推广。
Purification of human cord blood cord blood by potassium thiocyanate salting-out method to obtain partially purified interferon (P-IFN). The antiviral activity is about 50 times higher than that of crude interferon, and the specific activity is 10 to 5 units mg protein ~ (-1). The recovery rate is about 37%, more loss in the purification process. In order to reduce losses, in addition to taking care of the cold outside the operation, and to change dialysate dialysis, shorten the dialysis time, with cellulose microporous membrane instead of Chua’s filter, made some minor improvements. P-IFN treated by an ascending method had no significant effect on titer. 28 cases of herpetic keratitis were treated with subconjunctival or superficial orbital injection of P-IFN at a low concentration (1 ~ 3 × 10 ~ 4 units mL -1), with a cure rate of 92.9% The best skin type, the cure rate was 100%. This method has the advantages of high efficacy, small reaction, less dosage of interferon, which is good for promotion.