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This paper summarized the research advances of several commonly used alfalfa transformation technologies including Agrobacterium-mediated transformation method,electroporation method,microinjection method and biolistic method,and introduced the latest research findings in genetic improvement of alfalfa using transformation technologies to improve the quality and increase the resistance against diseases,insects and herbicides,as well as in production of plant-based vaccines,enzyme preparations and antibodies using transgenic alfalfa as biological reactors. In addition,the current bottleneck constraining the development of alfalfa transformation technologies and related biosafety issues were analyzed and discussed,and the application prospects and commercial value of transgenic alfalfa were proposed.
This paper summarized the research advances of several commonly used alfalfa transformation technologies including Agrobacterium-mediated transformation method, electroporation method, microinjection method and biolistic method, and the latest research findings in genetic improvement of alfaal using transformation technologies to improve the quality and increase the resistance against diseases, insects and herbicides, as well as in production of plant-based vaccines, enzyme preparations and antibodies using transgenic alfalfa as biological reactors. In addition, the current bottleneck constraining the development of alfalfa transformation technologies and related biosafety issues were analyzed and discussed, and the application prospects and commercial value of transgenic alfaalfa were proposed.