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对含有烟草花叶病毒番茄株系（TMV－L）部分基因组CDNA的克隆进行缺失改造，得到了含有TMV－L运动蛋白（MP）基因的克隆。MP片段分别与组成型启动子CaMV35S和病原特异诱导型启动子CHS、PiII和BG体外重组，构建成植物表达载体P35S－30K，pCHS－30K，pPiII－30K和pBG－30K。通过农杆菌LBA4404介导，分别转化含Tin－22抗性基因的番茄品种Geneva80。转化p35S－30K的基因工程植株在苗期观察到了部分坏死和黄化现象，初步证明TMV－LMP与Tm－22存在基因对基因的互作关系。诱导型启动子控制下的MP转基因番茄已得到部分潮霉素抗性愈伤组织，为进一步获得广谱高效抗病植株奠定了基础。
The clone containing part of the genomic CDNA of tobacco mosaic virus (TMV-L) was deleted and the clone containing the TMV-L motor protein (MP) gene was obtained. MP fragments were constructed into plant expression vectors P35S-30K, pCHS-30K, pPiII-30K and pBG-30K by in vitro recombination with constitutive promoter CaMV35S and pathogen-specific inducible promoters CHS, PiII and BG respectively. Agrobacterium tumefaciens LBA4404 was used to transform the tomato variety Geneva80 with the resistance gene Tin-22. Partial transformation of p35S-30K genetically engineered plants observed in the seedling stage of necrosis and yellowing, preliminary evidence of TMV-LMP and Tm-22 exist gene-gene interaction. MP transgenic tomato under the control of inducible promoter has obtained partial hygromycin-resistant callus, which lays the foundation for further obtaining broad-spectrum and high-efficient resistant plants.