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目的:观察吡格列酮对糖尿病大鼠认知功能及海马组织TNF-α、β-淀粉样蛋白(Aβ)的影响。方法:随机将45只Wistar大鼠分为正常对照组、糖尿病组、吡格列酮组,STZ25mg.kg-1腹腔注射建立糖尿病大鼠模型,给予吡格列酮10mg.kg-1.d-1灌胃干预12周后,采用Morris水迷宫试验测试其认知能力,ELISA法检测各组大鼠海马组织TNF-α和Aβ表达。结果:吡格列酮组与糖尿病组Morris水迷宫测试中逃避潜伏期分别较正常对照组明显延长(P<0.05);与糖尿病组比较,吡格列酮组Morris水迷宫测试中潜伏期明显缩短(P<0.05);中心区停留时间百分比和通过原平台位置次数增加(P<0.05);TNF-α表达吡格列酮组和糖尿病组与对照组相比明显升高(P<0.01);Aβ表达吡格列酮组和糖尿病组与对照组相比明显升高(P<0.01);吡格列酮组TNF-α和Aβ表达较糖尿病组降低(P<0.05)。结论:糖尿病大鼠认知功能受损;吡格列酮对糖尿病大鼠的认知功能减退具有改善作用,其机制可能与海马组织TNF-α、Aβ的表达降低有关。
Objective: To observe the effect of pioglitazone on the cognitive function and the levels of TNF-α and β-amyloid (Aβ) in hippocampus of diabetic rats. Methods: Forty-five Wistar rats were randomly divided into normal control group, diabetic group and pioglitazone group. STZ25mg.kg-1 was intraperitoneally injected to establish diabetic rat model. Pioglitazone 10mg.kg-1.d-1 was administered intragastrically for 12 weeks After that, Morris water maze test was used to test their cognitive ability, and ELISA was used to detect the expression of TNF-α and Aβ in hippocampus of rats in each group. Results: The escape latency of Morris water maze test in pioglitazone group and diabetic group was significantly longer than that in normal control group (P <0.05). Compared with diabetic group, the latent period of Morris water maze test in pioglitazone group was significantly shorter (P <0.05) (P <0.05). The expression of TNF-α in pioglitazone group and diabetic group was significantly higher than that in control group (P <0.01). The expression of Aβ in pioglitazone group and diabetic group was significantly higher than that in control group (P <0.01). The expressions of TNF-α and Aβ in pioglitazone group were lower than those in diabetic group (P <0.05). CONCLUSION: The cognitive function of diabetic rats is impaired. Pioglitazone can ameliorate the cognitive decline of diabetic rats, which may be related to the decrease of the expression of TNF-α and Aβ in hippocampus.