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病毒诱导基因沉默(virus-induced gene silencing,VIGS)技术,具有周期短、成本低、不需要转化遗传、高通量等优势,而被广泛地应用于探究植物生长发育、抗病及生理代谢等相关基因功能的鉴定。观赏植物具有较高的经济效益,VIGS技术在观赏植物中的应用具有良好的前景。现通过综述VIGS技术的建立与发展、作用机制及在观赏植物基因功能鉴定的应用实例,探讨该技术应用于观赏植物中影响沉默效率的因素;并展望其在观赏植物保护、丰富观赏性状的分子育种领域方面的应用前景。
Virus-induced gene silencing (VIGS) has been widely used in plant growth and development, disease resistance and physiological metabolism because of its short cycle, low cost, no need of genetic transformation, high-throughput and other advantages. Related gene function identification. Ornamental plants with high economic benefits, VIGS technology in ornamental plants has a good prospect. By reviewing the establishment and development of VIGS technology, the mechanism of action and the application of the appraisal of gene function in ornamental plants, this paper explores the factors that affect the efficiency of silencing ornamentation in ornamental plants, and looks forward to its application in the conservation of ornamental plants and the enrichment of ornamental traits Application prospect in breeding field.