论文部分内容阅读
目的:研究养血清脑颗粒对大鼠肝CYP1A2活性的影响。方法:实验分为空白对照、酶诱导、酶抑制及养血清脑组,以非那西丁作为探针药物。大鼠分别给予生理盐水、酶诱导剂、酶抑制剂和养血清脑颗粒,灌胃7天后,静脉注射非那西丁,不同时间点采集血样,用高效液相色谱法测定血浆中非那西丁的浓度,用Topfit 2.0软件估算其药动学参数。结果:空白对照、酶诱导、酶抑制和养血清脑组探针药物非那西丁的t1/2分别为(0.44±0.05)、(0.27±0.02)、(0.87±0.34)、(0.41±0.04)h;CL分别为(21.78±3.57)、(31.04±2.83)、(12.77±3.26)、(18.32±1.61)mL·min-1。结论:养血清脑颗粒对大鼠肝CYP1A2无明显的抑制或诱导作用。
Objective: To study the effect of Yangxue Qingnao Granule on the liver CYP1A2 activity in rats. Methods: The experiment was divided into blank control, enzyme induction, enzyme inhibition and Yangxue Qingnao group, phenacetin as a probe drug. Rats were given saline, enzyme inducer, enzyme inhibitor and nourishing qingnao granules, 7 days after gavage, intravenous phenacetin, blood samples taken at different time points, high performance liquid chromatography for the determination of phenacetin Ding concentration was estimated using Topfit 2.0 software pharmacokinetic parameters. Results: The values of t1 / 2 of phenacetin in the blank control, enzyme induction, enzyme inhibition and Yangxue Qingnao injection were (0.44 ± 0.05), (0.27 ± 0.02), (0.87 ± 0.34) and (0.41 ± 0.04 ) h; CL were (21.78 ± 3.57), (31.04 ± 2.83), (12.77 ± 3.26) and (18.32 ± 1.61) mL · min-1, respectively. Conclusion: Yangxue Qingnao Granule has no obvious inhibition or induction on rat liver CYP1A2.