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Aus373是典型的秋稻广亲和品种,曾有报道指出,Aus373广亲和基因位于第七染色体上,与RFLP标记RZ488、RG511连锁,该基因暂被命名为Sa(t)n[3]。在此基础上,该研究对Aus373广亲和基因在RFLP图谱上的定位作了初步尝试。分析了S5和Sa(t)两位点的遗传效应。在Aus373/轮回422的F2群体中,Sn5和Sa(t)n效应基本相同,表现为重复基因作用的特点。推测群体中出现的1/16半不育株,是这两个广亲和基因位点非亲和性基因互作的结果。借助于分子标记辅助选择,筛选了1株可能具有两个位点广亲和基因的纯合重组体。并对有关秋稻的广亲和基因、Sns与Sa(t)n之间的相互关系及Sa(t)n的应用前景等问题进行了分析和探讨
Aus373 is a typical broad-leaved variety of autumn rice. It has been reported that the Aus373 broad-compatibility gene is located on chromosome 7 and linked to RFLP markers RZ488 and RG511. The gene was temporarily named Sa (t) n [3]. On this basis, this study made a preliminary attempt to locate the Aus373 broad-compatibility gene on the RFLP map. The genetic effects of S5 and Sa (t) were analyzed. In the Aus37 / reincarnation 422 F2 population, the effects of Sn5 and Sa (t) n were essentially the same, showing the characteristics of repeated gene interactions. The estimated 1/16 semi-sterile line in the population is the result of the interaction between the two non-affinitive genes at the wide-compatibility locus. By means of molecular marker-assisted selection, a homozygous recombinant that may have a wide locus gene of two loci was screened. The relationship between Sans and Sa (t) n and the broad-compatibility genes of autumn rice and the prospects of its application to Sa (t) n were analyzed and discussed