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影响冻融胚胎移植结局的因素很多,包括患者年龄、冷冻胚胎的质量、内膜准备方式和冷冻方法等。传统的程序化冷冻在临床已使用20余年,虽经不断改进,解冻存活率仍在70%左右。玻璃化冷冻不需要程序化冷冻仪,且简便、快速,仅数分钟即可完成,消耗液氮量少,正逐渐广泛应用于辅助生殖实验室。数据显示玻璃化冷冻囊胚和卵子解冻后的存活率均高于慢速冷冻方法。
There are many factors affecting the outcome of frozen-thawed embryo transfer, including patient age, quality of frozen embryos, intimal preparation and freezing methods. The traditional programmed freezing has been used for more than 20 years in clinical practice. The survival rate of thawing is still about 70% after continuous improvement. Vitrification freezing does not require programmed freezing, and simple, fast, only a few minutes to complete, consume less liquid nitrogen, is gradually widely used in assisted reproductive laboratories. The data showed that the survival rates of vitrified blastocysts and eggs after thawing were higher than those of slow freezing methods.