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OBJECTIVE To investigate the effects and molecular mechanisms of Baicalin,a natural flavonoid compound derived from Scutellariabaicalensis Georgi,in the triple-negative human breast cancer cell line MDAMB-231.METHODS Cel s(1.0×105mL-1)were seeded in96-well plates,6-well plates or 25 cm2flasks.After overnight incubation,various concentrations of Baicalin were added to cells for another 48 h.Cell viability was measured using XTT Assay.Cell growth and migration was measured using colony formation assay and wound healing assay,autophagy-related proteins were observed using Western blotting analysis.RESULTS A dose-dependent decrease in cell viability was induced by Baicalin(IC50=48.6μg·m L-1).The colony-forming activity of MDA-MB-231 cells was significantly reduced by various concentrations of baicalin(25,50,100μg·m L-1)(85.2±12.7%,41.3±12.3%,19.6±6.6%).Wound healing assay showed that the recovery rateof baicalin-treated groups(25,50,100μg·m L-1)were significantly lower than those of the untreated group(88.3±15.1%,52.1±15.5%,28.3±9.6%).Western blot showed that the AMP-activated protein kinase and ULK1 was clearly up-regulated and activated by Baicalin(25,50,100μg·m L-1)in a dose-dependent manner,and the expression level of autophagic marker Beclin-1 and LC3A/B was also unregulated by the same treatment.CONCLUSION The study revealed that baicalin interferes with breast cancer growth by inducing autophagy,which at least in part through AMPK/ULK1 activation.
OBJECTIVE To investigate the effects and molecular mechanisms of Baicalin, a natural flavonoid compound derived from Scutellariabaicalensis Georgi, in the triple-negative human breast cancer cell line MDAMB-231. METHODS Cel s (1.0 x 105 mL-1) were seeded in 96-well plates , 6-well plates or 25 cm2 flasks. After overnight incubation, various concentrations of Baicalin were added to cells for another 48 h. Cell viability was measured using XTT Assay. Cell growth and migration was measured using colony formation assay and wound healing assay, autophagy -related proteins were observed using Western blotting analysis .RESULTS A dose-dependent decrease in cell viability was induced by Baicalin (IC50 = 48.6 μg · m L-1). The colony-forming activity of MDA-MB-231 cells was significantly reduced Wound healing assay showed that the recovery rate of baicalin-treated groups (25, 50, 100 μg · m L-1) (85.2 ± 12.7%, 41.3 ± 12.3%, 19.6 ± 6.6% · M L-1) were significantly lower than those of the untreated group (88.3 ± 15.1%, 52.1 ± 15.5%, 28.3 ± 9.6%). Western blot showed that the AMP-activated protein kinase and ULK1 was clearly up-regulated and activated by Baicalin (25, 50, 100 μg · m L -1) in a dose-dependent manner, and the expression level of autophagic marker Beclin-1 and LC3A / B was also unregulated by the same treatment. CONCLUSION The study revealed that baicalin interferes with breast cancer growth by inducing autophagy, which at least in part through AMPK / ULK1 activation.