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通过3年定位试验对不同培肥方式下黑土土壤中与氮素转化有关的功能菌动态变化进行了研究。结果表明,农肥处理和农肥化肥等量配比处理提高了土壤中好气性自生固氮菌数量,单施化肥的处理抑制了好气性自生固氮菌生长;农肥高量处理土壤中氨化细菌数量最高,是对照的108.87%,化肥低量处理氨化细菌数量最低,平均比对照高43.79%;农肥处理土壤亚硝酸盐细菌数量显著高于化肥处理,纯化肥处理土壤中反硝化细菌数量高于农肥处理,因此长期合理地施用农肥对降低土壤的反硝化作用,从而减少黑土氮素的损失有良好的作用。
Through 3-year experiment, the dynamic changes of nitrogen-related functional bacteria in black soil were studied under different fertilization methods. The results showed that the amount of aerobic Azotobacter in the soil increased with the equal proportion of fertilizer application and fertilizer treatment. The application of chemical fertilizer alone inhibited the growth of aerobic Azotobacter. The highest amount of ammoniated bacteria was found in the soil with high amount of agricultural fertilizer, Which is 108.87% of the control. The amount of ammoniated bacteria treated with a low amount of chemical fertilizer is the lowest, with an average of 43.79% higher than that of the control. The amount of nitrite bacteria in soil was significantly higher than that of chemical fertilizers, and the amount of denitrifying bacteria in pure fertilizers was higher than that of agricultural fertilizers Therefore, the long-term and reasonable application of agricultural fertilizer to reduce soil denitrification, thereby reducing the loss of black soil nitrogen has a good effect.