A rapid reverse dot blot assay for all 18 β-thalassemia mutations in Chinese population

• 作 者: 张基增 徐湘民 马维芳 单越新
• 机 构: DepartmentofBiochemistryMolecularBiologyLaboratory,FirstMilitaryMedicalUniversity.Guangzhou510515,De
• 出 处: Journal of Medical Colleges of PLA
• 发表日期: 1993年3期
• 关键词: Β-THALASSEMIA REVERSE DOT blot(RDB) gene diagnosis POLYMERASE chain reaction(PCR

A set of allele-specific oligonucleotide (ASO) probes used for detecting all 18 β-tha-lassemia mutations found in Chinese was immobilized on two strips of Biodyne C membrane;one containing 7 pairs of oligonucleotide probes specific for the most commonly f