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以含有1 mmol/L Al3+的S-LB培养基作为筛选培养基,从酸性土壤中分离到13株耐铝的细菌菌株,选取其中6株进行形态学分析,结果观察到这些菌株的菌体均呈杆状,其中1株为革兰阳性反应,其余5株为革兰阴性反应。以细菌通用引物扩增这些菌株的16S rDNA并测序,将得到的序列与GenBank中的序列进行BLAST比对,利用MEGA 4.0软件,按照Neighbor-joining法构建系统进化树,这6个菌株分别与Enterobacter endosymbiont、Serratia marces-cens、Pantoea agglomerans、Enterobacter aerogenes、Bacillus subtilis和Enterobacter asburiae的亲缘关系最近。将这些菌株接种到加有2 mmol/L Al3+、pH 4.5的S-LB固体培养基上培养时,它们都能生长,说明这些菌株具有较好的耐铝能力,这些菌株为进一步研究细菌的耐铝机制提供了极好的材料。
Thirteen strains of aluminum-tolerant bacteria were isolated from acidic soils using S-LB medium containing 1 mmol / L Al3 + as the screening medium. Six of them were selected for morphological analysis. As a result, the bacterial cells of these strains were observed Was rod-shaped, of which 1 was Gram-positive reaction, and the remaining 5 Gram-negative reaction. The 16S rDNAs of these strains were amplified by universal bacterial primers and sequenced. The obtained sequences were BLAST aligned with the sequences in GenBank. The phylogenetic tree was constructed by using MEGA 4.0 software according to the Neighbor-joining method. These 6 strains were respectively associated with Enterobacter endosymbiont, Serratia marces-cens, Pantoea agglomerans, Enterobacter aerogenes, Bacillus subtilis and Enterobacter asburiae. When these strains were inoculated into S-LB solid medium supplemented with 2 mmol / L Al3 + and pH 4.5, they all grew, indicating that these strains have good aluminum tolerance. These strains are further studies on bacterial resistance Aluminum mechanism provides excellent material.