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为探讨胎儿CMV感染产前诊断的可能性,作者自1985年10月至1992年7月检查了52例孕妇(40例血清CMV IgM阳性,12例有感染症状)。诊断手段包括每4周一次的超声检查,羊膜穿刺及胎儿血标本测定。特异性试验包括胎儿血清1:20稀释时特异性IgM抗体、病毒培养以及多聚酶链反应CMVDNA扩增,用Southern印迹法肯定诊断。非特异性试验包括白细胞计数、血红蛋白、血球容积、血小板等测定。在出生时对所有新生儿的尿和
To explore the possibility of prenatal diagnosis of fetal CMV infection, the authors examined 52 pregnant women (40 serum CMV IgM positive and 12 infected) from October 1985 to July 1992. Diagnostic tools include ultrasound every 4 weeks, amniocentesis and fetal blood samples. Specificity tests include specific IgM antibodies diluted 1: 20 in fetal serum, viral culture, and polymerase chain reaction (CMVDNA) amplification and positive diagnosis by Southern blotting. Non-specific tests include white blood cell count, hemoglobin, hematocrit, and platelet assays. Urine and urine for all newborns at birth