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目的:探讨商陆皂苷甲(EsA)对大鼠肾缺血再灌注(I/R)损伤的影响及机理。方法:SD大鼠60只,随机分为对照组(C组)、缺血再灌注组(I/R组)和缺血前EsA预处理组(EsA组),每组20只;采用夹闭双侧肾蒂30min后再灌注的方法 制备肾缺血再灌注损伤大鼠模型,检测大鼠血清和肾组织样本中血肌酐(SCr)、血尿素氮(BUN)、IL-17A、IL-6及钠-钾ATP酶水平,HE染色后评估肾小管损伤程度。结果:与对照组比较,EsA组大鼠术前摄水量增多、摄食减少(P<0.05);再灌注12h后,与对照组比较,I/R组大鼠SCr、BUN水平升高(P<0.05),EsA组无显著性差异(P>0.05);与对照组比较,I/R组大鼠肾组织IL-17A、IL-6表达水平升高(P<0.05),钠-钾ATP酶表达水平下降(P<0.05),EsA组无显著性差异(P>0.05);C组、I/R组和EsA组肾小管损伤评分为3.02±1.13、10.53±2.13和9.85±1.91,EsA组较I/R组评分降低(P<0.05)。结论:EsA预处理能降低大鼠肾缺血再灌注时的损伤程度,发挥保护作用,其机制与减少炎症因子释放、增加钠-钾ATP酶表达有关。
Aims: To investigate the effect and mechanism of Esculentoside A (EsA) on renal ischemia-reperfusion (I / R) injury in rats. Methods: Sixty SD rats were randomly divided into control group (C group), ischemia reperfusion group (I / R group) and pre-ischemic EsA pretreatment group (EsA group) The rat model of renal ischemia / reperfusion injury was established by reperfusion after 30min of bilateral renal pedicle. Serum creatinine (SCr), blood urea nitrogen (BUN), IL-17A and IL-6 And sodium - potassium ATPase levels, HE staining assessment of renal tubular injury. Results: Compared with the control group, the water intake of EsA group increased and the food intake decreased (P <0.05). Compared with the control group, the levels of SCr and BUN increased significantly in I / R group after reperfusion for 12 hours (P < 0.05). Compared with control group, the expression of IL-17A and IL-6 in renal tissue in I / R group was significantly increased (P <0.05) (P <0.05). The scores of renal tubule injury in group C, I / R and EsA were 3.02 ± 1.13, 10.53 ± 2.13 and 9.85 ± 1.91, respectively, while those in EsA group Compared with I / R group, scores decreased (P <0.05). CONCLUSION: EsA pretreatment can reduce the degree of renal ischemia-reperfusion injury and exert its protective effect. Its mechanism is related to reducing the release of inflammatory cytokines and increasing the expression of sodium-potassium ATPase.