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在抗生素研究或生產上,接種菌種所用的芽孢培養基是個關鍵問題。優越的培養基不特能使菌絲長得快。芽孢繁殖得好,而且經過接種,不發生變異现象。Moyer等氏的含有甘油蛋白腖的青黴菌芽孢培養基,Waksman氏的鏈黴菌固體培養基,均能滿足研究與生產抗生素的需要。但有些抗生素菌種對於生長芽孢條件的要求,就比較特殊,金黴菌就是一個例子。在1952年金黴素研究工作的伊始,我們曾用Dugger氏與Van Dyck等氏的芽孢培養基,接種一株由國外寄來的金黴素菌種,始終未見到芽孢的形成。有時僅能見到極少量的氣生菌絲,所以當時的研究工作就未能開展。我們曾以50%的蒸熟大米加3.5%瓊脂,作芽孢培養基,培育出金黴菌的芽孢,不
The spore culture medium used to inoculate the bacteria is a key issue in antibiotic research or production. Superior medium does not make mycelium grow faster. Spore propagation well, but after inoculation, no mutation occurs. Moyer’s Glycyrrhizin containing Penicillium spore culture medium, Waksman’s Streptomyces solid medium, can meet the needs of research and production of antibiotics. However, some antibiotic strains for the growth of spore conditions require a more special, Kimome is an example. At the beginning of the work on chlortetracycline in 1952, we used spore culture medium of Dugger’s and Van Dyck et al. Inoculated with a chlortetracycline strain sent abroad, we never found any sporulation. Sometimes only very few aerial mycelium can be seen, so the research work at that time failed to carry out. We used 50% steamed rice plus 3.5% agar as spore culture medium to grow spores of Chrysostomyum