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目的了解河南省流行的麻疹野病毒的基因特征,为制定消除麻疹策略提供依据。方法用非洲绿猴肾细胞/淋巴信号激活因子转染的非洲绿猴肾细胞[Vero Cell/Signaling Lymphocyte Activation Molecule(Vero/SLAM)]从疑似麻疹病例标本中分离麻疹野病毒,用逆转录-聚合酶链反应(Reverse Transcription-Polymerase ChainReaction,RT-PCR)扩增麻疹野病毒分离株的核蛋白(Nucleoprotein,N)基因羧基末端676个核苷酸片段,再对扩增产物进行核苷酸序列测定和分析。以N基因羧基末端450个核苷酸片段构建基因亲缘性关系树,进行核苷酸变异分析。结果河南省2008～2009年共分离麻疹病毒147株,挑选其中73株测序后均为H1基因型,73株麻疹野病毒450个核苷酸的平均进化距离为0.020。2008年分离株与参考毒株的平均进化距离为0.055,其中与Hunan.China93-7/H1基因型的进化距离为0.012～0.026;2009年分离株与参考毒株的平均进化距离为0.067,其中与H1基因型的进化距离为0.013～0.020。结论河南省2008～2009年麻疹野病毒流行优势株为H1基因型,未发现病毒有较大变异,但存在一定的进化距离,呈现多个传播链的流行。
Objective To understand the genetic characteristics of the wild measles wild virus in Henan Province and to provide evidence for the development of measles elimination strategy. Method / lymphoid African green monkey kidney cells transfected with signal activation factor [Vero Cell / Signaling Lymphocyte Activation Molecule (Vero / SLAM)] isolated measles viruses from samples suspected measles cases with African green monkey kidney cells, reverse transcription - polymerization The 676 nucleotides of the carboxyl end of Nucleoprotein (N) gene of wild measles virus were amplified by reverse transcription-polymerase chain reaction (RT-PCR), and the amplified products were sequenced And analysis. Nucleotide sequence analysis was performed by constructing a phylogenetic tree based on 450 nucleotides of the carboxyl terminus of N gene. Results A total of 147 strains of measles virus were isolated in Henan Province from 2008 to 2009, of which 73 were selected as H1 genotypes after sequencing, and the average evolutionary distance of 450 nucleotides of 73 wild measles virus strains was 0. 020. In 2008, The average evolutionary distance of the isolates was 0.055, of which the evolutionary distance from Hunan.China93-7 / H1 genotype was 0.012-0.0226. The average evolutionary distance between isolates and reference strains was 0.067 in 2009, of which the evolutionary distance from H1 genotype 0.013 ~ 0.020. Conclusion The predominant epidemic strain of wild measles virus from 2008 to 2009 in Henan province was H1 genotype. No significant variation was found in the virus, but there was a certain evolutionary distance and the prevalence of multiple transmission chains.