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目的探讨预先给予阿的平(quinacrine)对微波辐射致小鼠脑损伤的保护作用。方法小鼠随机分为4组,即正常组、辐射对照组、阿的平低剂量组(12.6 mg/kg)、阿的平高剂量组(50.4 mg/kg)。接受辐射动物于照射后即刻(0 d),1,2,7 d后完成检测,正常组动物在辐射实验后7 d后完成检测。微波辐照后,于不同时间点提取小鼠的脑组织蛋白,检测脑组织中HSP70蛋白表达变化。结果脑组织蛋白表达检测显示,微波辐射后1 d,辐射对照组小鼠脑中HSP70蛋白的表达开始升高,直至7 d。阿的平两个剂量组0,1,7 d给药组与辐射对照组相比明显上调,高剂量组尤甚。辐射对照组小鼠大脑海马的病理切片显示细胞水肿、血管扩张肿胀、间质充血等损伤,并出现海马沟回组织松散、细胞核皱缩、细胞水肿、空泡现象。预先灌胃给予小鼠阿的平后,小鼠脑组织的病理损伤有所减轻,状况有所改善。结论阿的平的这些保护机制可能与其上调HSP70蛋白表达发挥抗炎和抗凋亡作用,降低微波辐照引起的组织、细胞炎症反应和凋亡、坏死有关。
Objective To investigate the protective effect of quinacrine pretreatment on brain injury induced by microwave in mice. Methods The mice were randomly divided into 4 groups: normal control group, radiation control group, low dose albion group (12.6 mg / kg) and high dose albion group (50.4 mg / kg). Radiological animals received irradiation immediately (0 d), 1, 2, 7 d after completion of the test, the normal group of animals 7 days after the radiation test to complete the test. After microwave irradiation, the brain tissue proteins of mice were extracted at different time points to detect the expression of HSP70 protein in brain tissue. Results The protein expression of brain tissue showed that the expression of HSP70 protein in the brain of irradiated control mice began to increase up to 7 d after 1 d of microwave irradiation. A flat two dose group 0,1,7 d administration group and radiation control group was significantly higher, especially in the high-dose group. Pathological sections of the hippocampus of mice in the radiation control group showed cell edema, vasodilation and swelling, interstitial hyperemia and other injuries, and there was loose tissue, nuclear shrinkage, cell edema and vacuolization in the hippocampus. Pre-given intragastric administration of mouse A Ping, the mouse brain tissue pathological damage has been reduced, the situation improved. Conclusions These protective mechanisms of a-ping may be related to its anti-inflammatory and anti-apoptotic effects by up-regulating the expression of HSP70 protein, reducing the tissue-induced inflammation and apoptosis and necrosis induced by microwave irradiation.