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目的 :将多烯紫杉醇与顺铂以不同给药顺序处理人乳腺癌细胞研究两者细胞毒性的相互作用。方法 :应用一系列体外实验如DNA片段分析、MTT测定、流式细胞仪分析和蛋白印迹法分析两者以不同给药顺序的实验结果。结果 :显示当同时给药或顺铂在多烯紫杉醇前给药则发生拮抗作用 ;多烯紫杉醇在顺铂前给药则不发生拮抗作用。结论 :顺铂干扰多烯紫杉醇对癌细胞的捕获和细胞凋亡 ,并影响多烯紫杉醇诱导的bcl 2磷酸化 ;多烯紫杉醇与顺铂的相互作用具有高度给药顺序依赖性
OBJECTIVE: To study the cytotoxicity of docetaxel and cisplatin in human breast cancer cells treated by different administration sequences. Methods: A series of in vitro experiments such as DNA fragment analysis, MTT assay, flow cytometry and Western blotting were used to analyze the experimental results of the two drugs in different administration sequences. Results: Antagonism was shown when concurrent administration or cisplatin was given before docetaxel; docetaxel did not antagonize before cisplatin administration. CONCLUSIONS: Cisplatin interfered with the capture and apoptosis of docetaxel on cancer cells and the effect of docetaxel-induced bcl 2 phosphorylation. The interaction between docetaxel and cisplatin was highly order-dependent