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目的研究大鼠脑损伤后大脑皮质、海马和脑干中蛋白质表达的特点及差异。方法56只雄性SD大鼠,随机分为正常对照组、手术对照组及损伤组,后者于损伤后4 h、8 h、12 h、24 h和48 h不同时间取材观察。复制Marmarou’s落体打击脑损伤模型,应用弱阳离子交换芯片(WCX-2)和固定金属亲和吸附芯片(IMAC-Cu)结合表面增强激光解析电离飞行时间质谱技术分析大鼠闭合性脑损伤后皮质、海马和脑干中蛋白质表达谱的变化。结果(1)在WCX-2芯片上,大脑皮质中共捕获404个蛋白质峰;海马中共捕获364个蛋白质峰;脑干中共捕获495个蛋白质峰。在IMAC-Cu芯片上,皮质中共捕获203个蛋白质峰;海马中共捕获345个蛋白质峰;脑干中共捕获107个蛋白质峰。(2)与手术对照组相比,损伤组大脑皮质在两种芯片上共有38个蛋白质峰的相对强度差异有统计学意义(P<0.05);海马在两种芯片上共有49个蛋白质峰的相对强度差异有统计学意义(P<0.05);脑干在两种芯片上共有27个蛋白质峰的相对强度有显著性差异(P<0.05)。结论(1)脑损伤可引起不同脑区中蛋白质表达谱发生变化。(2)不同脑区蛋白质表达谱存在差异。
Objective To study the characteristics and differences of protein expression in cerebral cortex, hippocampus and brain stem after traumatic brain injury in rats. Methods Fifty-six male Sprague-Dawley rats were randomly divided into normal control group, operation control group and injury group. The latter group were observed at 4 h, 8 h, 12 h, 24 h and 48 h after injury. Marcarou’s model of brain injury was replicated and the cortical tissue of rats with closed brain injury were analyzed by WCX-2 and IMAC-Cu combined with surface enhanced laser desorption / ionization time-of-flight mass spectrometry. Changes in protein expression profiles in hippocampus and brain stem. Results (1) A total of 404 protein peaks were captured in the cerebral cortex on WCX-2 chip; 364 protein peaks were captured in the hippocampus; 495 protein peaks were captured in the brain stem. In the IMAC-Cu chip, a total of 203 protein peaks were captured in the cortex; a total of 345 protein peaks were captured in the hippocampus; a total of 107 protein peaks were captured in the brain stem. (2) Compared with the control group, there were statistically significant differences in the relative intensity of 38 protein peaks in the cerebral cortex between the two groups (P <0.05). There were 49 protein peaks in the hippocampus (P <0.05). There was a significant difference in the relative intensity of 27 protein peaks on the two kinds of chips (P <0.05). Conclusion (1) Brain injury can cause changes in protein expression profiles in different brain regions. (2) There are differences in protein expression profiles in different brain regions.