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目的 :观察睡眠剥夺后大鼠海马超微结构的病理变化及一氧化氮 (NO)含量、超氧化物歧化酶 (SOD)活力变化。方法 :以小平台水环境法建立大鼠睡眠剥夺模型 ,并设立大平台对照组 ,分离海马 ,电镜观察海马神经细胞超微结构变化、测定海马组织匀浆NO含量和SOD活力。结果 :睡眠剥夺组大鼠海马NO含量及SOD活力均高于大平台组和正常对照组 (P <0 0 5 )。病理形态学改变 ,光镜电镜下海马神经元减少 ,核仁碎裂、胞质内细胞器减少。结论 :睡眠剥夺可引起海马NO含量和SOD活力增高 ,并且存在病理形态学改变
Objective: To observe the pathological changes of hippocampal ultrastructure and the content of nitric oxide (NO) and the activity of superoxide dismutase (SOD) of rats after sleep deprivation. Methods: The rat sleep deprivation model was established by the small platform water environment method. A large platform control group was established. The hippocampus and the ultrastructure of the hippocampal neurons were observed under electron microscope. The contents of NO and SOD in hippocampus were measured. Results: The NO content and SOD activity of hippocampus in sleep deprivation group were significantly higher than those in large plateau group and normal control group (P <0.05). Pathological changes, under the light microscope electron microscopy decreased hippocampal neurons, nucleolus fragmentation, decreased cytoplasmic organelles. Conclusion: Sleep deprivation can induce NO increase in hippocampus and SOD activity, and there is pathological changes