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目的：探讨４℃条件下保存对血液氧化与抗氧化反应的影响。方法：采用化学比色法检测血浆丙二醛（ＭＤＡ）含量，血浆总氧抗氧化活性（ＡＯＡ）和红细胞超氧化物歧化酶（ＳＯＤ）活力。结果：血液在４℃保存时，随贮存时间的延长，血浆ＭＤＡ含量逐渐增加（Ｐ＜００５，Ｐ＜００１），血浆总抗化活性和红细胞ＳＯＤ活力逐渐降低，（Ｐ＜００５，Ｐ＜００１）。２１ｄ后红细胞膜的流动性明显降低（Ｐ＜００５，Ｐ＜００１）。结论：全血贮存过程中的氧化损伤，除与自由基在膜上发生过氧化反应作用外，还与抗氧化系统障碍有关。
Objective: To investigate the effect of 4 ℃ preservation on blood oxidation and anti-oxidation. Methods: Plasma malondialdehyde (MDA) content, plasma total oxygen antioxidant activity (AOA) and erythrocyte superoxide dismutase (SOD) activity were measured by chemical colorimetry. Results: When the blood was preserved at 4 ℃, the content of plasma MDA gradually increased with the prolongation of storage time (P <005, P <001), and the total plasma antiactivation activity and erythrocyte SOD activity decreased gradually (P <0 05, P <001). After 21 days, the fluidity of erythrocyte membrane decreased significantly (P <005, P <001). Conclusion: Oxidative damage during storage of whole blood is related to the anti-oxidative system disorder besides free radical peroxidation reaction on the membrane.