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目的研究中国浙江省道地药材浙贝母的遗传多样性。方法应用扩增片段长度多态性技术(AFLP)对具有代表性的6个浙贝母居群,共32份个体进行分析。结果在浙贝母的物种水平上,Nei′s基因多样性指数(He)为0.169 0±0.175 7,Shannon′s信息指数(I)为0.269 8±0.245 3,多态位点百分率(PPB)76.85%;总遗传多样性(Ht)0.169 0±0.030 9,其居群水平上遗传多样性(Hs)0.150 8±0.024 0,Shannon′s信息指数(I)0.233 3±0.261 9,多态位点百分率(PPB)50.38%,Nei′s遗传分化指数(Gst)为0.107 6,基因流(Nm)为4.147 0。东阳和永康种群之间的遗传距离最小(0.015 0),而象山与缙云最远(0.032 4)。结论浙江省主产区栽培浙贝母物种的遗传多样性水平丰富,高的遗传多样性能够维持浙贝母的长期生存,居群间的多样性水平明显低于居群内的多样性水平,居群间遗传分化不明显,种植资源相对比较稳定,这些居群适宜作为浙贝母优良品种的选育基地。
Objective To study the genetic diversity of Fritillaria cirrhosae, a native medicinal material in Zhejiang Province, China. Methods A total of 32 individuals of 6 Fritillaria cirrhosa populations were analyzed by using amplified fragment length polymorphism (AFLP). Results The Nei’s gene diversity index (He) was 0.169 0 ± 0.175 7, Shannon’s information index (I) was 0.269 8 ± 0.245 3, the percentage of polymorphic loci (PPB) 76.85%, total genetic diversity (Ht) 0.169 0 ± 0.030 9, population genetic diversity (Hs) 0.150 8 ± 0.024 0, Shannon’s information index (I) 0.233 3 ± 0.261 9, polymorphism Point percentage (PPB) was 50.38%, Nei’s genetic differentiation index (Gst) was 0.107 6, gene flow (Nm) was 4.147 0. The genetic distance between Dongyang and Yongkang was the smallest (0.015 0), while the distance between Xiangshan and Jinyun was the longest (0.032 4). Conclusion The genetic diversity of Fritillaria cirrhosa Cultivated in the main producing areas of Zhejiang Province is rich. The high genetic diversity can maintain the long-term survival of Fritillaria cirrhosa. The diversity level among the populations is obviously lower than that within the population, The genetic differentiation among populations is not obvious, and the planting resources are relatively stable. These populations are suitable breeding bases for fine varieties of Fritillaria cirrhosa.