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作者研究了尿激酶(UK)水溶液在不同的 pH(5.0～8.0)、散射光(1000 lux)或暗处、有或无亚硫酸氢钠(SBS,1.0×10~(-3)～3.0×10~(-3)mol/L)下的稳定性。发现随着亚硫酸氢钠的浓度增加 UK 的剩余活性减少。含1.0×10~(-2)mol/L SBS 的UK(120 IU/ml),25℃ 4h 后 UK 剩余活性为初始时的86.6％(散射光)和89.0％(暗处),而不含 SBS 的 UK,贮存6h(无论散射光或暗处)后失活均不明显。含 SBS 的UK 初始失活过程,在散射光或暗处均服从伪一级反应。在 pH 为5.0～8.0范围内,含 SBS的 UK 稳定性随 pH 值的增加而提高。UK 在pH5.0散射光下,加入 SBS(1.0×10~(-3)3mol/L)其剩余活性明显下降。但含 SBS的 UK 在暗处的稳定性比散射光下要好,尤其在 pH 5.0时。这种由于散射光和暗处间UK 剩余活性的差异可能是由于散射光照射后,亚硫酸盐的自氧化作用形成的游离基所
The authors studied the effects of aqueous urokinase (UK) solutions with or without sodium bisulfite (SBS, 1.0 × 10 -3 -3 ×) at different pH (5.0-8.0), scattered light 10 ~ (-3) mol / L). The residual activity of UK was found to decrease with increasing sodium bisulfite concentration. UK (120 IU / ml) containing 1.0 × 10 -2 mol / L SBS showed a UK residual activity of 86.6% (scattered light) and 89.0% (dark place) at initial after 4 h at 25 ° C, SBS UK, storage 6h (regardless of scattered light or dark place) after inactivation are not obvious. Initial inactivation of UK with SBS obeys pseudo-first order reaction in scattered light or in darkness. The stability of UK with SBS increased with the increase of pH value in the range of pH 5.0 ~ 8.0. UK at pH5.0 scattered light, the addition of SBS (1.0 × 10 ~ (-3) 3mol / L) its residual activity decreased significantly. However, UK with SBS is more stable in the dark than scattered light, especially at pH 5.0. This difference in residual activity between the scattered light and the dark UK may be due to the formation of free radicals by the self-oxidation of sulfite after the scattered light is irradiated