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目的探讨 5氟尿嘧啶 (5 Fu)诱导肝癌细胞凋亡与caspase 8活性变化的关系。方法采用caspase 8荧光检测试剂盒检测HepG2细胞凋亡过程中caspase 8活性变化。流式细胞仪检测加入caspase 8抑制剂IETD FMK后 5 Fu诱导的HepG2细胞凋亡百分率的变化。结果不同浓度的 5 Fu均可诱导HepG2细胞caspase 8活性升高 ,高浓度与低浓度比较差异有显著意义 (P <0 0 0 1)。肝癌细胞的caspase 8活性随 5 Fu作用时间延长而逐步升高 ,至 16h后达到高峰。IETD FMK能阻断caspase 8活化而抑制 5 Fu诱导的HepG2细胞凋亡。结论 5 Fu通过caspase 8信号传导通路诱导肝癌细胞凋亡 ;5 Fu诱导caspase 8活性升高有浓度与时间依赖性
Objective To investigate the relationship between apoptosis induced by 5-Fu and changes of caspase 8 activity in hepatocellular carcinoma cells. Methods Caspase 8 fluorescence detection kit was used to detect the changes of caspase 8 activity during HepG2 cell apoptosis. Flow cytometry was used to detect the percentage of apoptosis induced by 5 Fu in HepG2 cells after adding IETD FMK, a caspase 8 inhibitor. Results Different concentrations of 5 Fu could induce caspase 8 activity in HepG2 cells. There was significant difference between high concentration and low concentration (P <0.01). The activity of caspase 8 in hepatocarcinoma cells gradually increased with the prolongation of 5 Fu, reaching the peak after 16 h. IETD FMK blocked caspase 8 activation and inhibited 5 Fu-induced HepG2 cell apoptosis. Conclusion 5 Fu induces apoptosis of hepatocellular carcinoma cells through caspase 8 signal transduction pathway. 5 Fu induces caspase 8 activity in a concentration-and time-dependent manner