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陆生植物叶绿体RNA编辑是转录后基因表达调控的一种重要方式。该文在预测棉花(Gossypium hirsutum)叶绿体基因RNA编辑位点的基础上,选取中棉10(CRRI 10)为实验材料,采用PCR、RT-PCR及测序等方法,确定CRRI 10的27个叶绿体蛋白编码基因共有55个编辑位点,均是C→U的转换。与棉种柯字310(C310)的编辑位点比对后发现,CRRI 10多出accD-468和rpoC1-163两个编辑位点,同时缺失psbN-10。利用生物信息学分析这3个位点,rpoC1-163和psbN-10的编辑可能会改变各自蛋白的二级结构。对CRRI 10中55个编辑位点上游的顺式作用元件(?30–?1)分析显示,共有8组顺式作用元件的相似性达到60%或以上,推测各组中的编辑位点可能由相同的反式作用因子来识别。
Terrestrial Plant Chloroplast RNA Editing is an Important Way of Regulation of Transcriptional Gene Expression. Based on the prediction of RNA editing sites of chloroplast genes in cotton (Gossypium hirsutum), we selected CRIM 10 as the experimental material and determined the chloroplast proteins of 27 CRRI 10 by PCR, RT-PCR and sequencing, A total of 55 coding genes editing sites, are C → U conversion. Comparisons with the editing sites of cotton seed Kexiao 310 (C310) revealed that CRRI 10 contained two more editing sites, accD-468 and rpoC1-163, with deletion of psbN-10. Using bioinformatics analysis of these three sites, editing of rpoC1-163 and psbN-10 may alter the secondary structure of the respective proteins. Analysis of the cis-acting elements (? 30-? 1) upstream of the 55 editing sites in CRRI 10 revealed that the similarity of the cis-acting elements in all 8 groups reached 60% or more, suggesting that the editing sites in each group may Identified by the same trans-acting factor.