论文部分内容阅读
目的:建立了苦参药材中10种黄酮类成分(染料木苷,三叶豆紫檀苷,马卡因,异黄腐醇,苦参醇I,槐黄醇,苦参酮,降苦参酮,2-O-甲基苦参酮及异苦参酮)HPLC测定方法,并对野生与栽培苦参黄酮类成分含量进行比较研究。方法:色谱条件为Kromasil C_(18)色谱柱(4.6 mm×250 mm,5μm),甲醇(A)-0.3%甲酸水(B)为流动相进行梯度洗脱(0~5 min,50%A;5~25 min,50%~70%A;25~35 min,70%A;35~50 min,70%~90%A;50~55 min,90%A),流速0.8 m L·min~(-1),检测波长设定295 nm,数据处理采用独立样本t检验,辅以聚类分析(HCA)和主成分分析(PCA)方法进行评价。结果:独立样本t检验显示,各类成分均表现为P>0.05,即野生品与栽培品在黄酮类含量上无差异;聚类分析和主成分分析也表现为栽培品与野生品苦参黄酮类成分无差异。方法学考察表明10种黄酮类成分具有较好的线性,在其线性范围内r值均>0.999。平均加样回收率在95.0%~105.0%,RSD均在5%以内,符合含量测定的要求。结论:该测定方法快速、准确,可为苦参的质量控制方法提供参考。
OBJECTIVE: To establish a method for the determination of 10 flavonoid components in genus Sophora flavescens (genistin, trifoliate paliperidin, macacaine, isoflavone, sophora alcohol I, sophorol, matrine, , 2-O-methyltromethorphan and isoflavone), and compared the contents of flavonoids in wild and cultivated Sophora flavescens. Methods: The chromatographic conditions were gradient elution (0-5 min, 50% A) with Kromasil C 18 column (4.6 mm × 250 mm, 5 μm) and methanol (A) -0.3% ; 5 to 25 min, 50 to 70% A; 25 to 35 min, 70% A; 35 to 50 min, 70 to 90% A; ~ (-1). The detection wavelength was set at 295 nm. The data was processed by independent sample t-test, and the results were analyzed by clustering analysis (HCA) and principal component analysis (PCA). Results: Independent sample t-test showed that all kinds of ingredients showed P> 0.05, that is, there was no difference in the content of flavonoids between wild and cultivated products. Cluster analysis and principal component analysis also showed that the content of flavonoids No difference in class composition. Methodological study showed that 10 kinds of flavonoids have good linearity, in the linear range of r values were> 0.999. The average recoveries ranged from 95.0% to 105.0% with RSD within 5%, which met the requirements of determination of content. Conclusion: The method is rapid and accurate and can provide a reference for the quality control of Sophora flavescens.