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水稻稻瘟病是最具毁灭性的病害之一,严重地影响水稻的高产和稳产。在病原菌侵染水稻时,附着胞的形成对稻瘟病菌的致病性起关键作用。研究证实一种P型ATP酶(PATPase)参与了附着胞的形成。在病原菌与寄主的互作过程中,寄主的一些小分子物质可以进入病原菌中,达到抗病原菌侵染的目的。本研究以稻瘟菌致病关键的P-ATPase基因MgAPT2第一外显子上特异性好的232 bp区域作为干扰片段,正反向插入干涉载体中,通过农杆菌介导,转化到感稻瘟病水稻品种日本晴中,通过苗期稻瘟病接种鉴定和MgAPT2基因的表达检测,结果表明:转基因植株稻瘟病抗性得到增强且稻瘟病菌MgAPT2基因的表达量下降。该研究为水稻抗稻瘟病种质资源的创新提供了新思路。
Rice blast is one of the most devastating diseases, seriously affecting the high yield and stable yield of rice. In the pathogen infection of rice, the formation of appressorium plays a key role in the pathogenicity of Magnaporthe grisea. Studies confirm that a P-type ATPase (PATPase) is involved in the formation of appressorium. In the course of the interaction between the pathogen and the host, some small molecules of the host can enter the pathogen to achieve the purpose of anti-pathogen infection. In this study, a specific 232 bp region of the first exon of MgAPT2, the key P-ATPase gene responsible for the pathogenesis of Magnaporthe grisea, was used as an interference fragment and inserted into the interference vector in both forward and reverse directions. The Agrobacterium tumefaciens-mediated transformation into sense rice The blast rice variety Nipponbare was identified by blast inoculation at seedling stage and the expression of MgAPT2 gene was detected. The results showed that the blast resistance of transgenic plants was enhanced and the expression level of MgAPT2 gene of Magnaporthe grisea decreased. The research provided a new idea for the innovation of rice blast resistance germplasm resources.