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采用分子杂交、PCR-SSCP技术检测了39例膀胱癌及7例正常膀胱组织中HPV16、18感染及Ha-ras、p53、RB基因变异,并结合临床分期、病理分级等参数进行了综合分析.结果表明,膀胱癌组织中HPV16、18感染率及Ha-ras、p53、RB基因变异率分别为61.5%、36%、30.8%及15.4%;HPV阳性率与分级分期、p53基因突变与HPV感染呈明显负相关;Ha-ras、p53基因突变率与分级分期正相关,其中Ha-ras基因在复发肿瘤的突变率显著高于原发肿瘤;部分标本同时呈HPV感染与p53基因突变、Ha-ras与p53基因突变.提示高危HPV感染和Ha-ras、p53、RB基因变异及相互作用在很大程度上参与了膀胱癌的发生及演变,并与其生物学行为密切相关,有望成为判断肿瘤恶性程度、自然病程及预后的估价指标.
HPV16,18 infection and the genetic variations of Ha-ras, p53 and RB in 39 cases of bladder cancer and 7 cases of normal bladder tissue were detected by molecular hybridization and PCR-SSCP, and analyzed by clinical stage, pathological grade and other parameters. The results showed that the infection rate of HPV16,18 and the mutation rates of Ha-ras, p53 and RB in bladder cancer tissues were 61.5%, 36%, 30.8% and 15.4% respectively. The positive rates of HPV and grading, p53 gene mutation and HPV infection The mutation rate of Ha-ras and p53 was positively correlated with the grading stage. The mutation rate of Ha-ras gene in recurrent tumors was significantly higher than that of the primary tumor. Some of the samples were HPV infection and p53 gene mutation, Ha- ras and p53 gene mutation.It is suggested that the high risk HPV infection and the gene mutation and interaction of Ha-ras, p53, RB are involved in the occurrence and evolution of bladder cancer to a large extent and are closely related to their biological behavior, Degree, the natural course of disease and prognosis of the indicators.