血液细胞化学用于先天性代谢缺陷患者及携带者的检出与诊断的研究

• 作 者: 李成林 黄世琪 王建军 王俊梅 刘玉荷 郝奋
• 机 构: 包头医学院优生研究室!014010,包头医学院优生研究室!014010,包头医学院优生研究室!014010,包头医学院优生研究室!014010,包头医学院优生研究室!014010,包头医学院优生研究室
• 出 处: 中国优生与遗传杂志
• 发表日期: 1997年06期
• 关键词: 血液细胞 先天性代谢缺陷 携带者 髓性过氧化物酶 磷酸葡萄糖 黄嘌呤氧化酶 定量技术 过氧化氢酶 显示方法 细胞化学技术

为探索血液细胞化学技术用于先天性代谢缺陷的检查与诊断，本文研究了血细胞中与代谢缺陷有关的酶．并对各种酶的显示方法．反应物的形态、分布，进行了观察与记述，同时还应用半定量技术对酶反应强度进行分级，取得了各种酶活性的正常值，从而为实际应用提供一些有价值的基础资料。本文首先报告下列一些酶：（1）过氧化氢酶，（2）髓性过氧化物酶，（3）黄嘌呤氧化酶，（4）乳酸脱氢酶，（5）谷氨酸脱氢酶，（6）NADH脱氢酶，（7）NADPH脱氢酶，（8）醇脱氢酶，（9）6—磷酸葡萄糖脱氢酶，（10）6—磷酸葡萄糖酸脱氢酶，（11）3β—羟基类固醇脱氢酸，（12）3—磷酸甘油醛脱氢酶，（13）木糖醇脱氢酶，（14）醛缩酶，（15）发酸酐酶，（16）磷酸葡萄糖异构酶。

In order to explore the use of hematocytochemistry in the detection and diagnosis of congenital metabolic defects, we investigated the enzymes involved in metabolic defects in blood cells. And a variety of enzyme display method. The morphology and distribution of the reactants were observed and described. At the same time, semi-quantitative techniques were also used to classify the reaction intensities of enzymes, and the normal values ​​of various enzyme activities were obtained. Thus, some valuable basic data were provided for practical application. This paper first reports on the following enzymes: (1) catalase, (2) myeloperoxidase, (3) xanthine oxidase, (4) lactate dehydrogenase, (5) glutamate dehydrogenase (6) NADH dehydrogenase, (7) NADPH dehydrogenase, (8) alcohol dehydrogenase, (9) 6-phosphogluconate dehydrogenase, (12) glyceraldehyde 3-phosphate dehydrogenase, (13) xylitol dehydrogenase, (14) aldolase, (15) hair acid anhydrase, (16) phosphoglucose Isomerase.