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我们利用体外培养的正常人肾小球系膜细胞,观察了纯化的人低密度脂蛋白和高密度脂蛋白对该细胞产生血小板活化因子的影响。结果发现:经刺激20h,在一定浓度范围内,低密度脂蛋白能增加培养系膜细胞上清液中血小板活化因子的含量,且在浓度为100μg/ml时,培养细胞上清液中血小板活化因子的上升显著,在200μg/ml时达高峰。而高密度脂蛋白则仅在高浓度(350μg/ml)时有轻度刺激作用。这些结果说明:低密度脂蛋白具有刺激肾小球系膜细胞产生血小板活化因子的作用。
We use the normal human mesangial cells cultured in vitro to observe the purified human low density lipoprotein and high density lipoprotein on the platelet-activating factor. The results showed that LDL increased the content of platelet-activating factor in the culture supernatant of mesangial cells at a certain concentration range after stimulated for 20 h, and at the concentration of 100 μg / ml, platelet activation in the supernatant The rise of the factor was significant, reaching a peak at 200 μg / ml. High-density lipoproteins, on the other hand, showed mild irritation only at high concentrations (350 μg / ml). These results indicate that LDL has the effect of stimulating the production of platelet-activating factor by mesangial cells.