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目的:观察大鼠纹状体主神经元、快速放电中间神经元及低阈值放电中间神经元的电学特性。方法:应用脑片膜片钳全细胞记录技术,在大鼠纹状体脑片标本上,监测及观察纹状体内神经元的被动和主动电学特性以及动作电位的发放特征。结果:纹状体主神经元静息膜电位处于轻度超极化状态,具有内向整流特性,并且在触发第一个动作电位之前有一个相对缓慢的去极化过程;纹状体快速放电中间神经元的放电频率很高,其动作电位具有较大幅度的后超极化,去极化电流可以诱导该神经元产生快速放电并伴随阈下膜电位震荡;纹状体低阈值放电中间神经元具有较大的输入阻抗和绝对值较小的静息膜电位,这类神经元具有低阈值放电的典型电学特性。结论:大鼠纹状体主神经元和中间神经元的被动和主动电学特性存在明显差异,这可以作为区分不同类型神经元的依据。
OBJECTIVE: To observe the electrical properties of rat striatum main neurons, rapidly discharging interneurons and low threshold discharge interneurons. Methods: Whole-cell patch-clamp technique was used to monitor and observe the passive and active electrical properties of neurons in the striatum and the distribution of action potentials in rat striatum slices. Results: The resting membrane potential of the striatum main neuron was in a mildly hyperpolarized state with an inward rectifying property and a relatively slow depolarization process before the first action potential was triggered. In the striatum rapid discharge center The discharge frequency of neurons is very high, and their action potentials have more significant post-hyperpolarization. Depolarization current can induce rapid discharge of the neurons with concussion of subthreshold membrane potential. The striatal low threshold discharge interneurons With greater input impedance and smaller resting absolute membrane potential, these neurons have the typical electrical properties of low threshold discharge. CONCLUSION: There are significant differences in the passive and active electrical characteristics between the main neurons and the interneurons of striatum in rats, which can be used as the basis for differentiating different types of neurons.