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目的：探讨ｎｍ２３－Ｈ１基因表达与大肠癌转移的关系。方法：应用过氧化酶标记的链霉卵白素（ＳＰ）染色法对３３例正常大肠粘膜、１３４例癌旁粘膜及１９３例大肠癌组织进行免疫组织化学染色，并对染色结果进行观察。结果：大肠粘膜与癌旁粘膜间ｎｍ２３－Ｈ１表达阳性率差异无显著性（Ｐ＞０．０５），但ｎｍ２３－Ｈ１在良、恶性大肠癌组织中的表达阳性率差异有显著性（Ｐ＜０．０５～０．０１）。ｎｍ２３－Ｈ１表达阳性率仅在正常、良性大肠组织与转移的大肠癌间差异有显著性（Ｐ＜０．０１），但与未转移大肠癌间差异无显著性（Ｐ＞０．０５）。在已发生转移的大肠癌组织中，ｎｍ２３－Ｈ１表达阳性率和强度明显低于尚未发生转移者（Ｐ＜０．０１）。在３９例无淋巴结转移的大肠癌组织中未见ｎｍ２３－Ｈ１表达，而在２４例转移的大肠癌中可检测到ｎｍ２３－Ｈ１表达。结论：ｎｍ２３－Ｈ１在抑制大肠癌转移中发挥一定作用，但在大肠癌转移过程中可能还有其他调节途径／因素存在。
Objective: To investigate the relationship between nm23-H1 gene expression and metastasis of colorectal cancer. METHODS: Immunohistochemical staining of 33 cases of normal colorectal mucosa, 134 cases of paraneoplastic mucosa and 193 cases of colorectal cancer tissues were performed using peroxidase-labeled streptavidin (SP) staining method. The staining results were observed. Results: There was no significant difference in the expression of nm23-H1 between colorectal mucosa and paratumor mucosa (P>0.05). However, the positive expression rate of nm23-H1 in benign and malignant colorectal cancer tissues was significantly different (P< 0.05 to 0.01). The positive rate of nm23-H1 expression was only significant between normal and benign colorectal tissues and metastatic colorectal cancer (P<0.01), but there was no significant difference between normal and benign large intestine tissue and metastatic colorectal cancer (P>0.05). In metastatic colorectal cancer tissues, the positive rate and intensity of nm23-H1 expression were significantly lower than those without metastasis (P<0.01). There was no expression of nm23-H1 in 39 colorectal cancer tissues without lymph node metastasis, but nm23-H1 expression was detected in 24 metastatic colorectal cancers. Conclusion: nm23-H1 plays a role in the inhibition of metastasis of colorectal cancer, but there may be other regulatory pathways/factors in the metastasis of colorectal cancer.