A versatile biosensing platform coupling CRISPR-Cas12a and aptamers for detection of diverse analyte

• 作 者: Xiangxiang Zhao Shanshan Li Guang Liu Zhong Wang Zhiheng Yang Quanwei Zhang Mindong Liang Jiakun Liu Zilong Li Yaojun Tong Guoliang Zhu Xinye Wang Lan Jiang Weishan Wang Gao-Yi Tan Lixin Zhang
• 机 构: State Key Laboratory of Bioreactor Engineering,and School of Biotechnology,East China University of
• 出 处: 科学通报（英文版）
• 发表日期: 2021年1期
• 关键词: CRISPR-Cas12a Aptamer Biosensing platform Diverse analyte Alpha fetoprotein dete

Rapid and sensitive detection of various analytes is in high demand.Apart from its application in genome editing,CRISPR-Cas also shows promises in nucleic acid detection applications.To further exploit the potential of CRISPR-Cas for detection of diverse analytes,we present a versatile biosensing platform that couples the excellent affinity of aptamers for broad-range analytes with the collateral single-strand DNA cleavage activity of CRISPR-Cas12a.We demonstrated that the biosensors developed by this platform can be used to detect protein and small molecule in human serum with a complicated background,i.e,the tumor marker alpha fetoprotein and cocaine with the detection limits of 0.07 fmol/L and 0.34 μmol/L,respectively,highlighting the advantages of simplicity,sensitivity,short detection time,and low cost compared with the state-of-the-art biosensing approaches.Altogether,this biosensing platform with plug-and-play design show great potential in the detection of diverse analytes.