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目的了解清远地区路氏锥虫人群和宿主动物感染情况。方法 2009年在连州市、2010年在佛冈县和清新县、2011年在阳山县和清城区的居民区(自然村)室内、外环境分别采用笼(夹)日法捕鼠(计算鼠密度);另外采用布电猫网和挖鼠洞等多种方式捕捉活鼠。对各种方法所捕获的全部活鼠取血液涂片染色镜检路氏锥虫感染情况。在调查地区随机采集健康人群血清不少于50份,采用双抗体夹心酶联免疫法(ELISA)测定血清标本中人路氏锥虫抗体。结果在5个调查地区共检测健康人血清270份,路氏锥虫抗体阳性率为25.93%(70/270),其中,北部地区(连州和阳山)人群血清路氏锥虫抗体阳性率为24.55%(27/110),南部地区(佛冈、清新和清城)人群血清路氏锥虫抗体阳性率为26.81%(43/160)(P>0.05)。调查地区的总鼠密度为8.11%(314/3 874),其中,室内环境家栖鼠密度为8.40%(198/2 358),室外环境野栖鼠密度为7.65%(116/1 516)。共涂片检查活鼠385只,路氏锥虫总感染率为12.99%(50/385);其中,家栖鼠类(褐家鼠、黄胸鼠和小家鼠)共285只(其中褐家鼠262只),感染率为14.39%(41/285)(其中褐家鼠感染率为13.74%);野栖鼠类(黄毛鼠、板齿鼠和臭鼩鼱)100只,感染率为9.00%(9/100)。结论清远地区的健康人群的路氏锥虫抗体水平较高,宿主动物以家栖鼠类为主,褐家鼠是带虫优势鼠种。建议在春秋季节鼠类活动高峰期开展灭鼠工作,同时临床上要注意对可疑病例的排查。
Objective To understand the prevalence of Trypanosoma cruzi and host animals in Qingyuan area. Methods In 2009, in Lianzhou City, in Fogang County and Qingxin County in 2010, indoor and outdoor environments in Yangshan County and Qingcheng District in 2011 were caged (trapped) Density); In addition to using cloth cat network and digging holes and other ways to capture live rats. All live mice captured by various methods were stained for smear-negative T. smegmatis infection. In the survey area randomly selected healthy population serum of not less than 50, using double antibody sandwich enzyme-linked immunosorbent assay (ELISA) Serum samples of Trypanosoma rerio antibodies. Results Serum samples of 270 healthy volunteers were detected in 5 investigated areas. The positive rate of Trypanosoma brucei antibody was 25.93% (70/270). The positive rate of Trypanosoma brucei antibody in the northern area (Lianzhou and Yangshan) Was 24.55% (27/110). The positive rate of Trypanosoma brucei antibody in the southern areas (Fogang, Qingxin and Qingcheng) was 26.81% (43/160) (P> 0.05). The total rodent densities in the surveyed areas were 8.11% (314/3 874), with 8.40% (198/2358) for indoor environment and 7.65% (116/1 516) for outdoor environment. A total of 385 live rats were examined by smear, and the total infection rate of Trypsinolates was 12.99% (50/385). Among them, 285 (Rattus norvegicus, Rattus flavipectus and Mus musculus) 262 domestic rats), the infection rate was 14.39% (41/285), of which 13.74% were infected by Rattus norvegicus; 100 wild rats (Rattus flavipectus, Banded rat and Smelly), the infection rate 9.00% (9/100). Conclusion Trypanosoma brucei antibodies are higher in healthy subjects in Qingyuan. The host animals are mainly domestic animals. Rattus norvegicus is the predominant species of rodents. It is recommended to carry out rodent control during the peak season of rodents in spring and autumn, and to pay attention to the investigation of suspicious cases clinically.