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目的了解北京市密云地区沙门菌的耐药特点和分子机制,并进行同源性分析。为沙门菌感染食源性疾病的防控提供科学依据。方法 2015年分离食源性疾患患者的沙门菌共28株,进行血清学鉴定和体外药敏试验,采用PCR扩增沙门菌的喹诺酮耐药区(QRDRs)基因(gyr A、gyr B、par C和par E),用脉冲场凝胶电泳(PFGE)检测菌株间的分子同源性。结果 2015年密云地区沙门菌感染主要以鼠伤寒沙门菌和都柏林沙门菌为主,通过PFGE图谱分型并聚类分析,分别分成7种和5种型别,喹诺酮耐药率最高,并以87位Asp→Tyr/Asn/Gly突变为主。结论密云地区食源性疾患患者沙门菌可能存在暴发,临床需谨慎使用喹诺酮类药物治疗沙门菌食源性疾患感染。
Objective To understand the drug resistance characteristics and molecular mechanisms of Salmonella in Miyun district of Beijing and to analyze the homology. To provide a scientific basis for the prevention and control of foodborne diseases caused by Salmonella infection. Methods A total of 28 Salmonella strains from patients with food-borne diseases were enrolled in this study. Serological and in vitro susceptibility tests were carried out. The genes encoding quinolone resistance region (QRDRs) of Salmonella were amplified by PCR (gyr A, gyr B, par C And par E), the molecular homology between strains was examined by pulsed-field gel electrophoresis (PFGE). Results In 2015, Salmonella infections in Miyun were mainly Salmonella typhimurium and Salmonella dulcis. According to the PFGE pattern classification and cluster analysis, they were divided into seven types and five types respectively, and the quinolone resistance rate was the highest. Asp → Tyr / Asn / Gly mutated to the main. Conclusion Salmonella in food-borne disease patients may be outbreak in Miyun area. Quinolone-based drugs should be used cautiously to treat food-borne salmonellosis in Miyun area.